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EBER ISH Epstein-Barr encoding region (EBER) in situ hybridization

EBER ISH Epstein-Barr encoding region (EBER) in situ hybridization

EBV probe,Epstein-Barr encoding region (EBER) in situ hybridization is the methodology of choice for the detection of the Epstein-Barr virus (EBV) in tissue sections.

Epstein-Barr virus (EBV), also known as human herpesvirus 4, is a member of the herpes virus family. It was the first oncogenic virus to be discovered. Infection by this virus can show signs of a slight viral infection or it can be present as Infectious Mononucleosis. The most common target cells for the Epstein-Barr virus are the B lymphocytes and the nasopharyngeal epithelial cells. The Epstein-Barr virus massively infects the human population and sero-epidemiological studies show that 90% of adults have been infected by this virus. There are numerous human tumors associated with EBV. These range from non-differentiated nasopharyngeal carcinoma to African Burkitts lymphoma, Hodgkins disease mixed cellularity, some B, T and NK lymphomas, as well as in lymphoproliferative processes associated with immunodeficiency. The EBV Probe is intended to be used for the qualitative detection of human Epstein-Barr virus (EBV) EBER RNA in formalin-fixed, paraffin-embedded specimens, by chromogenic in situ hybridization (CISH). A positive reactivity for Epstein-Barr-virus (EBV) EBER RNA in the target cells is indicated by a distinctly stained nucleus. Colored precipitates, which can be clearly distinguished from the background, will be dark brown when using DAB for detection.

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