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EGFR Antibody Reagent for Immunohistochemistry

EGFR Antibody Reagent for Immunohistochemistry

EGFR Antibody reagent——Primary Antibody


  • Positive Control:

    Skin
  • Cellular Localization:

    Cytoplasm/cell membrane
  • Application:

    IHC-P
  • Secondary Antibody:

    iVision™
  • Specification/ml:

    1、3、6、0.2(Concentrated)

 Product name

EGFR Antibody reagent——Primary Antibody

Intended use

For Research use only. EGFR Antibody reagent is intended for use to qualitatively identify EGFR by microscopy in sections of FFPE tissue using immunohistochemical detection system.

Principle

EGFR is a member of type I growth factor receptor family, which is composed of an extracellular functional domain that can bind epidermal growth factor, a short transmembrane domain and an intracellular domain with tyrosine kinase activity. EGFR is expressed in many normal tissues, especially in the basal layer of stratified epithelium and squamous epithelium. EGFR overexpression is found in many kinds of tumor tissues, such as breast, bladder, lung, stomach, esophagus and head and neck tumors. Add the primary antibody to bind the antigen on tissue sections, and then use HRP labeled secondary antibody binding primary antibody to form the secondary antibody- primary antibody- antigen complex. When DAB chromogenic solution is added, HRP reacts with enzyme substrate to produce brown insoluble reaction product, which indirectly indicating the existence of antigen. 

Main components

Immunoglobulin, antibody diluent

Storage

Store at 2~8℃ for 18 months

Sample requirements

FFPE tissues are usually cut into sections as thin as 35μm with a microtome. These sections are then mounted onto glass slides that are coated with a tissue adhesive.

Protocol

1. Sample preparationDeparaffinize the slides in xylene , , Ⅲ for 5 minutesTransfer the slides once through 100%, 100%, 95%, 75% alcohols for 2 minutes respectively. Rinse slides with deionized water for 30 seconds.

2BlockingBlock endogenous peroxidase activity by incubating sections in 3% H2O2 solution at room temperature for 5 minutes to block endogenous peroxidase activity. Rinse the slides with deionized water for 30 seconds.

3Antigen retrievalHeat the EDTA Antigen retrieval buffer to 100. Then place the slides in the boiled buffer and continue to heat for 1520 min. Naturally cool down for 30 minutes. Rinse the sample with wash buffer.

4Primary antibody incubation: Drain the slides. Add primary antibody to tissue, incubate at room temperature for 30 minutes. (use antibody diluent or PBS as control). Wash the slides in PBST for 2 times, 5 minutes for each time. If the Primary antibody is concentrated, please dilute it to RTU(ready to use) according to the information on packing. 

5. Secondary antibody: Drain the slides. Add secondary antibody to tissue and incubate at room temperature for 20 minutes. Wash the slides in PBST for 2 times, 5 minutes for each time.

6. DABDrain the slides. Add DAB to the tissue and incubate at room temperature for 5 min. Rinse slides with deionized water.

7. Hematoxylin stainingDrain the slides. Add Hematoxylin to the tissue and incubate at room temperature for 5 minutes. Rinse slides with water. Use the acid solution for differentiation. Rinse slides with water.

8. DehydrateDehydrate the slides in 75%, 95%, 100% alcohols for 2 minutes. Dry the slides. Cover stained tissue with a coverslip using mounting medium.

Positive localization

1. Positive localization: cell membrane/cytoplasm.

2. Positive control: placenta tissue.

Precautions

1. Please read the instruction carefully and become familiar with all components of the kit prior to use, Strictly follow the instruction during operation.

2. DO NOT use the kit or any kit component after their .

3. Only trained professionals can use this kit. Please wear suitable lab coat and disposable gloves while handling the reagents.

4. Avoid contact of skin, eyes and mucous membranes with the chemicals.

5. DO NOT pipet by mouth.

6. Unused reagents, used kit and waste must be disposed according to local regulations.

Manufacturer

Company Name: Xiamen Talent Biomedical Technology Co.,Ltd

Address: No.3rd and 4th Floors , Building B10, No. 2068 Wengjiao Road West, BioMedical Park, Haicang District, Xiamen City, 36100 China

Tel: +86 592 6315755            

E-mail: tlsw@talentbiomedical.com                

Website: www.talentbiomedical.com

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